We’d like to share a notable case where a highly toxic gene was successfully harnessed through precise host engineering—showcasing how challenging biological parts can be transformed into functional tools.
 
Case: ΦX174 Lytic Gene E
Gene E encodes a potent lytic protein that cleaves bacterial cell walls, causing 100% cell lysis.
Cloning this gene in standard E. coli strains failed due to leaky expression and interaction with the host protein SlyD, leading to premature cell death.
Small colonies and escape colonies ( large colonies )

Solution: Engineering a Neutral Host
By knocking out slyD, we created a safe cloning host where Gene E could be stably maintained. When transferred into SlyD-expressing strains, the gene functioned as intended, enabling controlled bacterial lysis.
 
Applications Include:
✔ Rapid protein release for purification
✔ Programmable plasmid elimination in synthetic biology
✔ Novel antibacterial strategies
 
The Precision Alliance of Synthesis & Editing
Gene Editing: Custom knockouts, point mutations, and knock-ins.
Gene Synthesis: High-accuracy synthesis of toxic, repetitive, or long DNA sequences.
Integrated Solutions:End-to-end support from design to functional chassis development.
 
Contact Us

 
Looking for more? These services might be just what you need:
Oligo Synthesis Protein Expression Antibody Services
 
 
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